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Molecule of the Month


162: Dermcidin

Author: David S. Goodsell

Viruses come in many shapes and sizes, ranging from simple protein shells filled with RNA or DNA, to membrane-enveloped particles that rival cells in complexity. HIV is one of these complex viruses, surrounded by a membrane and filled with a diverse collection of viral and cellular molecules. The genome of HIV, which is composed of two strands of RNA, is packaged inside a distinctive cone-shaped capsid, which protects the RNA and delivers it to the cells that HIV infects.

Staying Flexible

The HIV capsid is built from a single protein, called capsid protein, shown here from PDB entry 1e6j. Capsid protein, also known as CA or p24, folds to form two domains connected by a flexible linker. This flexibility gives the protein a lot of options for assembly. The larger domain associates with other copies of the protein to form rings of six, and slightly less often, rings of five. The smaller domain then links these rings together to form the larger structure.

Breaking Symmetry

The flexibility allows the formation of structures that aren't as perfectly symmetrical as the capsids of viruses like poliovirus or rhinovirus. Instead, HIV capsid forms an unusual cone-shaped structure, with twelve of the pentameric rings (shown here in orange) and over a hundred hexamers (shown here in red). This model was constructed based on electron micrographs, using PDB entries 3h47, 3p05, 1a43 and 2kod.

Fighting Back

TRIM5 is one of the weapons deployed in the ongoing battle is being fought between living organisms and viruses. TRIM5 binds to the retroviral capsid and interferes with the uncoating process. At this point in our evolution, the human version of TRIM5 is not effective against HIV, but it blocks other retroviruses. However, the TRIM5 protein from rhesus monkeys, shown here from PDB entry 3uv9, is highly potent against HIV. Another cellular protein is hijacked by HIV to assist in viral replication. As HIV is budding from an infected cell, the cellular enzyme cyclophilin A binds to capsid, as shown here from PDB entry 1ak4. Researchers are still working out its function in the viral lifecycle, but it seems to be essential for the proper uncoating of the virus when it infects a new cell.

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The capsid protein is able to form hexamers and pentamers by shifting slightly in structure. This is an example of the principle of "quasiequivalence", first proposed by Caspar and Klug in 1962. This is the way that many viruses build capsids using a single type of protein subunit, but much larger than is possible with perfect symmetry. In the HIV capsid, the interactions between the many subunits are similar, but are deformed slightly to accommodate the different shapes of the cone-shaped portion and the round caps. To take a closer look at these two structures, PDB entries 3mge and 3p05, click on the image for an interactive Jmol.

理解を深めるためのトピックス

  1. you can explore the structure of capsid and other HIV proteins in the online Flash animation: The Structural Biology of HIV.
  2. to explore the flexibility of capsid, you can use the Compare Structures tool at the RCSB PDB to overlap the different structures.
  3. in order to solve the structures of the hexagonal and pentagonal complexes, researchers engineered capsid with cysteines to lock the structure together. You can view these with the "View in 3D" Jmol viewer in entries 3mge or 3p05, by typing "select cys;spacefill;color yellow" in the "Scripting Options" input box (and be sure to hit the submit button) .

参考文献

代表的な構造

1e6j: HIV capsid and antibody Fab
3mge: HIV capsid hexamer
A cone-shaped capsid protects the HIV genome in the mature virus, and delivers it to the cells that HIV infects. This structure includes a hexamer of capsid proteins.
3p05: HIV capsid pentamer
A cone-shaped capsid protects the HIV genome in the mature virus, and delivers it to the cells that HIV infects. This structure includes a pentamer of capsid proteins.